A facile methodology successful in acquiring a set of compounds www.selleckchem.com/products/CHIR-258.html monofluorinated at different positions (fluorine scan) by chemical synthesis is reported. Direct and nonselective fluorination reactions of our lead compound la and critical intermediate 2a worked efficiently selleck inhibitor to afford a complete of 6 monofluorinated derivatives. Each of the derivatives kept their physicochemical properties in contrast with all the lead la and 1 of them had enhanced Raf/MEK inhibitory action. Holding physicochemical properties may be regarded a benefit of monofluorinated derivatives compared with chlorinated derivatives, iodinated derivatives, methylated derivatives, and so forth. This vital discovering led on the identification of compound 14d, which had potent tumor growth inhibition within a xenograft model, superb PKFerroptosis profiles in 3 animal species, and no important toxicity.